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C H A P T E R 4 Genetics

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  1. The emergence of antibiotic-resistant bacteria, especially in enteric

gram-negative rods, is a medically important phenomenon. This

most commonly occurs by a process that involves a sex pilus and

the subsequent transfer of plasmids carrying one or more transposons.

Which one of the following is the name that best describes

this process?

(A) Conjugation

(B) Transduction

(C) Transformation

(D) Translocation

(E) Transposition

 

  1. Several important pathogenic bacteria have the ability to translocate

pieces of their DNA in a process called programmed rearrangements.

Which one of the following is the most important

known consequence of this ability?

(A) The number of plasmids increases significantly, which greatly

enhances antibiotic resistance.

(B) The amount of endotoxin increases significantly, which greatly

enhances the ability to cause septic shock.

(C) The surface antigens of the bacteria vary significantly, which

greatly enhances the ability to avoid opsonization by antibody.

(D) The ability of the bacterium to be lysogenized is significantly

increased, which greatly enhances the ability to produce

increased amounts of exotoxins.

(E) The ability of the bacterium to survive intracellularly is greatly

increased.

 

  1. Which statement is the most accurate regarding transposons?

(A) They encode enzymes that degrade the ends of the bacterial

chromosome.

(B) They are short sequences of DNA that often encode enzymes

that mediate antibiotic resistance.

(C) They are short sequences of RNA that silence specific regulatory

genes.

(D) They are a family of transfer RNAs that enhance mutations at

“hot spots” in the bacterial genome.

 

  1. Corynebacterium diphtheriae causes the disease diphtheria by producing

diphtheria toxin. The gene encoding the toxin is integrated

into bacterial genome during lysogenic conversion. The toxin gene

was acquired by which process?

(A) Conjugation

(B) Transduction

(C) Transformation

(D) Translocation

(E) Transposition

✅ Answers (1)

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Private answer

(1) (A)

Bacterial conjugation is the bacterial equivalent of sexual reproduction or mating. To perform conjugation, one bacterium has to carry a transferable plasmid (referred to as either an F+ or an R+ plasmid), while the other must not. The transfer of plasmid DNA occurs from the F-positive bacterial cell to the F-negative bacterium (making it F+ once transfer is complete).

 

 

(2) (C)

Gene rearrangement is a phenomenon in which a programmed DNA recombination event occurs during cellular differentiation to reconstitute a functional gene from gene segments separated in the genome

 

 

(3) (B)

The transposons present into the bacteria are DNA transposons contain transposase and antibiotic resistance coding genes.

 

 

 

(4) (B)

When phages insert into the bacterial genome genes that encode toxins like Panton-Valentine, Shiga and diphtheria toxins other virulence factors, further excision may be aberrant, leading the phage genome to carry those virulence genes by transduction.

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Answered on June 24, 2020 5:01 pm

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