Lab assignment

Which of the following modifications is least likely to alter the rate at which a DNA fragment moves through a gel during electrophoresis?

Altering the nucleotide sequence of the DNA fragment.

Increasing the length of the DNA fragment - As the length of DNA fragment increases, the speed at the which it moves through the gel decreases.

Changing the electrical charges within the DNA fragment - When the charge within the DNA fragment is increased, the rate of movement towards the electrode is high.

Altering the nucleotide sequence of the DNA fragment - The sequence of the DNA does not determine the rate of movement through the gel. All nucleotides are negatively charged which means there is no significant impact in rate of movement.

What is a bacterial plasmid?

A piece of bacterial DNA that is separate from the chromosome.

In addition to chromosome, bacteria contain plasmids which are circular DNA molecules. These plasmids contains genes essential for survival of the bacteria, such as genes responsible for antibiotic resistance ability.

Which of the following modifications is least likely to alter the rate at which a DNA fragment moves through a gel during electrophoresis?

Altering the nucleotide sequence of the DNA fragment.

Increasing the length of the DNA fragment - As the length of DNA fragment increases, the speed at the which it moves through the gel decreases.

Changing the electrical charges within the DNA fragment - When the charge within the DNA fragment is increased, the rate of movement towards the electrode is high.

Altering the nucleotide sequence of the DNA fragment - The sequence of the DNA does not determine the rate of movement through the gel. All nucleotides are negatively charged which means there is no significant impact in rate of movement.

What is a bacterial plasmid?

A piece of bacterial DNA that is separate from the chromosome.

In addition to chromosome, bacteria contain plasmids which are circular DNA molecules. These plasmids contains genes essential for survival of the bacteria, such as genes responsible for antibiotic resistance ability.